Limited proteolysis of the beta-hexosaminidase precursor in a cell-free system.
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چکیده
منابع مشابه
Limited Proteolysis of the /&Hexosaminidase Precursor in a Cell-free System*
Pulse-chase experiments had shown that @-hexosaminidase was synthesized in cultured human fibroblasts in precursor form and that during maturation of the enzyme the a-chains were converted from Mr = 67,000 to 54,000 and the /&chains from M, = 63,000 to 29,000 plus smaller fragments, probably through an intermediate form of M, = 52,000 (Hasilik, A., and Neufeld, E. F. (1980) J. Biol. Chem. 255,4...
متن کاملSynthesis and assembly of a catalytically active lysosomal enzyme, beta-hexosaminidase B, in a cell-free system.
The synthesis and dimerization of beta-chains during the formation of catalytically active beta-hexosaminidase B were studied in a cell-free system. beta-chain mRNA, transcribed from the cloned cDNA with SP6 polymerase, was translated in a rabbit reticulocyte protein-synthesizing system in the presence of dog pancreas microsomal membranes and oxidized glutathione. Under these conditions, the pr...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43415-1